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clk 040 05  (Jena Bioscience)


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    Structured Review

    Jena Bioscience clk 040 05
    Clk 040 05, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bdp-fl-peg4-dbco/BDP-FL-PEG4-DBCO/pmc13627705-487-0--1
    Average 94 stars, based on 16 article reviews
    clk 040 05 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Monitoring Sphingomyelin Biosynthesis at Nanoscale Resolution by Expansion Microscopy
    Article Snippet: PrePrint: ing buffer for 1 h/RT. Samples were fixed with 4% PFA in PBS for 10 min/RT. Subsequently, samples were washed thrice and incubated with 0.5 µM (conventional microscopy) or 4 µM (4xExM) BODIPY-FL-DBCO (Jena Biosciences, Cat. No. CLK-040-05) in HBSS (Gibco™, Cat. No. 14025-100) for 1 h/37°C at 170 rpm. Samples were washed five times and incubated with 5 µM (conventional) or 20 µM (4xExM) AZDye546-azi

    Incubation:

    Article Title: Monitoring Sphingomyelin Biosynthesis at Nanoscale Resolution by Expansion Microscopy
    Article Snippet: PrePrint: ing buffer for 1 h/RT. Samples were fixed with 4% PFA in PBS for 10 min/RT. Subsequently, samples were washed thrice and incubated with 0.5 µM (conventional microscopy) or 4 µM (4xExM) BODIPY-FL-DBCO (Jena Biosciences, Cat. No. CLK-040-05) in HBSS (Gibco™, Cat. No. 14025-100) for 1 h/37°C at 170 rpm. Samples were washed five times and incubated with 5 µM (conventional) or 20 µM (4xExM) AZDye546-azi

    Blocking Assay:

    Article Title: Monitoring Sphingomyelin Biosynthesis at Nanoscale Resolution by Expansion Microscopy
    Article Snippet: PrePrint: ing buffer for 1 h/RT. Samples were fixed with 4% PFA in PBS for 10 min/RT. Subsequently, samples were washed thrice and incubated with 0.5 µM (conventional microscopy) or 4 µM (4xExM) BODIPY-FL-DBCO (Jena Biosciences, Cat. No. CLK-040-05) in HBSS (Gibco™, Cat. No. 14025-100) for 1 h/37°C at 170 rpm. Samples were washed five times and incubated with 5 µM (conventional) or 20 µM (4xExM) AZDye546-azi

    Saline:

    Article Title: Monitoring Sphingomyelin Biosynthesis at Nanoscale Resolution by Expansion Microscopy
    Article Snippet: PrePrint: ing buffer for 1 h/RT. Samples were fixed with 4% PFA in PBS for 10 min/RT. Subsequently, samples were washed thrice and incubated with 0.5 µM (conventional microscopy) or 4 µM (4xExM) BODIPY-FL-DBCO (Jena Biosciences, Cat. No. CLK-040-05) in HBSS (Gibco™, Cat. No. 14025-100) for 1 h/37°C at 170 rpm. Samples were washed five times and incubated with 5 µM (conventional) or 20 µM (4xExM) AZDye546-azi

    Staining:

    Article Title: Monitoring Sphingomyelin Biosynthesis at Nanoscale Resolution by Expansion Microscopy
    Article Snippet: PrePrint: ing buffer for 1 h/RT. Samples were fixed with 4% PFA in PBS for 10 min/RT. Subsequently, samples were washed thrice and incubated with 0.5 µM (conventional microscopy) or 4 µM (4xExM) BODIPY-FL-DBCO (Jena Biosciences, Cat. No. CLK-040-05) in HBSS (Gibco™, Cat. No. 14025-100) for 1 h/37°C at 170 rpm. Samples were washed five times and incubated with 5 µM (conventional) or 20 µM (4xExM) AZDye546-azi



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    Image Search Results


    (a-e) HeLa cells were incubated with α-NH 2 -ω-N 3 -C 6 -Cer and ppCho and visualized by 4xExM. Donor intensity before (pre) and after (post) bleaching the acceptor fluorophore was compared in whole cells (a) or indicated cellular compartments (b-e). (f) FRET efficiency in cellular compartments was determined and normalized to the FRET efficiency detected in the whole cell (set to 1, f). n=3. Scale bar (corrected for 4-fold expansion factor): 5 µm (a) and 1 µm (b-e). Statistics: One-way ANOVA and Tukey’s multiple comparison (f).

    Journal: bioRxiv

    Article Title: Monitoring Sphingomyelin Biosynthesis at Nanoscale Resolution by Expansion Microscopy

    doi: 10.64898/2026.05.27.726241

    Figure Lengend Snippet: (a-e) HeLa cells were incubated with α-NH 2 -ω-N 3 -C 6 -Cer and ppCho and visualized by 4xExM. Donor intensity before (pre) and after (post) bleaching the acceptor fluorophore was compared in whole cells (a) or indicated cellular compartments (b-e). (f) FRET efficiency in cellular compartments was determined and normalized to the FRET efficiency detected in the whole cell (set to 1, f). n=3. Scale bar (corrected for 4-fold expansion factor): 5 µm (a) and 1 µm (b-e). Statistics: One-way ANOVA and Tukey’s multiple comparison (f).

    Article Snippet: Subsequently, samples were washed thrice and incubated with 0.5 μM (conventional microscopy) or 4 μM (4xExM) BODIPY-FL-DBCO (Jena Biosciences, Cat. No. CLK-040-05) in HBSS (GibcoTM, Cat. No. 14025-100) for 1 h/37°C at 170 rpm.

    Techniques: Incubation, Comparison